Difference between revisions of "Part:BBa K5492203"
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Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1]. | Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1]. | ||
| − | + | This part uses [https://parts.igem.org/Part:BBa_K5492100 BBa_K5492100] designed for the conservation of sequences during synthesis and PCR reactions. | |
| + | Adapter sequences from Twist Bioscience are added to the 5' and 3' end of the part. | ||
| + | The primers for this sequence can be found here: [https://parts.igem.org/Part:BBa_K5492010 BBa_K5492010] [https://parts.igem.org/Part:BBa_K5492011 BBa_K5492011] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases. | Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases. | ||
Revision as of 20:05, 25 September 2024
Optimised DAO - pETDUET-1
Optimised coding sequence of rat Diamine Oxidase appropiated for insertion into pETDUET-1. This part uses BBa_K5492100 designed for the conservation of sequences during synthesis and PCR reactions. Adapter sequences from Twist Bioscience are added to the 5' and 3' end of the part. The primers for this sequence can be found here: BBa_K5492010 BBa_K5492011
Usage and Biology
Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine, and the resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 129
Illegal PstI site found at 190 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 129
Illegal BglII site found at 2392
Illegal BamHI site found at 123
Illegal BamHI site found at 2067
Illegal BamHI site found at 2321
Illegal XhoI site found at 937
Illegal XhoI site found at 2441 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190
Illegal NgoMIV site found at 2411 - 1000COMPATIBLE WITH RFC[1000]