Difference between revisions of "Part:BBa K5492101"
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<partinfo>BBa_K5492101 short</partinfo>
 
<partinfo>BBa_K5492101 short</partinfo>
  
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pETDUET-1 plasmid used for expression of Histamine-N-Methyltransferase and Diamine Oxidase in multiple e.coli chassis.
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pETDUET-1 plasmid used for expression of Histamine-N-Methyltransferase [https://parts.igem.org/Part:BBa_K5492400 BBa_K5492400], [https://parts.igem.org/Part:BBa_K5492401 BBa_K5492401] and Diamine Oxidase [https://parts.igem.org/Part:BBa_K5492200 BBa_K5492200] in multiple e.coli chassis.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:54, 25 September 2024


pETDUET-1

pETDUET-1 plasmid used for expression of Histamine-N-Methyltransferase BBa_K5492400, BBa_K5492401 and Diamine Oxidase BBa_K5492200 in multiple e.coli chassis.

Sequence and Features

https://static.igem.wiki/teams/5492/registry/petduet-map.webp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1256
    Illegal SapI.rc site found at 2916