Difference between revisions of "Part:BBa K5036015"

Revision as of 16:54, 25 September 2024

Nanobody1

Part Description

nanobodies are a unique type of antibody derived from camelids. These single-domain antibodies are significantly smaller and more stable, making them ideal for applications in diagnostics and therapeutics. Nanobodies can be used to develop highly sensitive and specific tests for a variety of diseases, including cancer and autoimmune disorders. Their compact size and stability also make them promising candidates for targeted drug delivery and other biomedical applications

Usage

we insert nanobodies to reach the conditioned circular RNA by mediating the attachment between NSP3 and the MCP of the aptamer

Dry lab Characterization

We Started by making a decision about which Nanobodies are candidates to be used in our project. We had to nominate 2 of 3 available nanobodies for the MMP-9 so we started by validating the nanobodies binding affinity to the MMP-9

MMP9-NB1 complex

This figure confirmed that The first nanobody-MMP9 interaction scored a binding stability (ΔG) of -12.3 kcal mol-1 which is considered a high binding stability .

Then we compared the binding stability between different nanobodies to MMP9

This figure shows that NB1 and NB3 have higher affinity over the NB2 which make them candidates to be used in our switch .

Sequence and Features