Difference between revisions of "Part:BBa K243011:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K243011 short</partinfo>
 
<partinfo>BBa_K243011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The choice of the linker, lipocalin tag and purification tag allows us serval different combinations
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The cloning steps were planed theoretically before we started the work in the wet lab.
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[https://static.igem.org/mediawiki/parts/2/24/Freiburg09_Strediglonglifoki.txt Commented GenBank file]
  
 
===Source===
 
===Source===

Revision as of 17:26, 21 October 2009

Strep-DigA-Long Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning steps were planed theoretically before we started the work in the wet lab.

Commented GenBank file

Source

Combined the parts by serial cloning steps.

References