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Revision as of 08:27, 25 September 2024
Vector-ZaTdT-KanR
Here, we loaded the genes encoding TdT into plasmid vectors. In this way we were able to over-express the ZaTdT in the Escherichia coli BL21.
Description
We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.
