Difference between revisions of "Part:BBa K5392015"

 
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Here, we loaded the genes encoding TdT into plasmid vectors. In this way we were able to over-express the ZaTdT in the Escherichia coli BL21.
 
Here, we loaded the genes encoding TdT into plasmid vectors. In this way we were able to over-express the ZaTdT in the Escherichia coli BL21.
  
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==Description==
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===Usage and Biology===
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We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.
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<span class='h3bb'>Sequence and Features</span>
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<img src="https://static.igem.wiki/teams/5392/wtzatdt.png" width="350" height="400"/>
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<partinfo>BBa_K5392015 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K5392015 parameters</partinfo>
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Revision as of 08:24, 25 September 2024


Vector-ZaTdT-KanR

Here, we loaded the genes encoding TdT into plasmid vectors. In this way we were able to over-express the ZaTdT in the Escherichia coli BL21.

Description

We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.