Difference between revisions of "Part:BBa K5115077"
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__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | This composite part is composed of nikA and improved ribozyme-assisted polycistronic co-expression system: pRAP. The nikA is a subunit of the the NikABCDE transporter, an ATP-binding cassette, made up by nikA, nikB, nikC, nikD and nikE. This composite part will finally be used in [https://parts.igem.org/Part:BBa_K5115082 BBa_K5115082(ribozyme connected nik operon)] for further experiment. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM]. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 05:34, 25 September 2024
ribozyme+RBS+nikA+stem-loop
Introduction
This composite part is composed of nikA and improved ribozyme-assisted polycistronic co-expression system: pRAP. The nikA is a subunit of the the NikABCDE transporter, an ATP-binding cassette, made up by nikA, nikB, nikC, nikD and nikE. This composite part will finally be used in BBa_K5115082(ribozyme connected nik operon) for further experiment.
Usage and Biology
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1635
- 1000COMPATIBLE WITH RFC[1000]