Difference between revisions of "Part:BBa K191005"

Latest revision as of 17:13, 21 October 2009

TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term

Part BBa_K191005 is a double-repressor system: there is a Trp promoter in front of the TetR gene, followed by the Tet promoter in front of the RFP gene. This means that it should react both to tryptophan and tetracycline, an allosteric blocker of TetR (for our experiments we used anhydrous tetracycline, ATC).

Usage and Biology

This part permits characterization of part BBa_K191003 as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see here.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
Illegal AgeI site found at 1576
Illegal AgeI site found at 1688
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K191005 short</partinfo>
-TRP inhibits TetR's transcription while TetR inhibits RFP's transcription.
+Part BBa_K191005 is a double-repressor system: there is a Trp promoter in front of the TetR gene, followed by the Tet promoter in front of the RFP gene. This means that it should react both to tryptophan and tetracycline, an allosteric blocker of TetR (for our experiments we used anhydrous tetracycline, ATC).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part permits characterization of part <partinfo>BBa_K191003</partinfo> as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see [https://parts.igem.org/Part:BBa_K191003 here].
 <!-- -->