Difference between revisions of "Part:BBa K2619004"
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| − | + | ==Usage and Biology== | |
| + | ====Team UVU-Utah-2, 2024==== | ||
| + | We obtained this part in the Chlamydomonas MoClo kit as described in Crozet et al. (2018). We utilized and characterized this part in composite part(s) K5078008. Please check out the documentation for that composite part for more details! | ||
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Revision as of 03:39, 25 September 2024
19kDa nano luciferase/nanoLuc
this Nano lucierase (nluc) is the mutated catalytic 19 kDa component of Oplophorus luciferase. nluc is a newly developed luciferase. In our project, it can be expressed as an indicator of exosome quantity by linking it with CD63 and lamp2b. We use it as reporter of membrane protein gene expression. It is also combined with C/D box and 3 stem loop to exam the transportation efficiency of packaging devices.
Usage and Biology
Team UVU-Utah-2, 2024
We obtained this part in the Chlamydomonas MoClo kit as described in Crozet et al. (2018). We utilized and characterized this part in composite part(s) K5078008. Please check out the documentation for that composite part for more details!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]