Difference between revisions of "Part:BBa K4770013"
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<partinfo>BBa_K4770013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4770013 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Add more about the biology of this part here --> | ||
| + | ===Usage and Biology=== | ||
| + | ====Team UVU-Utah-2, 2024==== | ||
| + | We obtained this part in the Chlamydomonas MoClo kit as described in Crozet et al. (2018). We utilized and characterized this part in composite part(s) K5078005, K5078006. Please check out the documentation for that composite part for more details! | ||
Latest revision as of 03:34, 25 September 2024
mCherry_Gene
Description and Biology
Red Fluorescent Protein mCherry is a constitutively monomeric red fluorescent tetrameric protein original from Discosoma sp. (sea anemones). Generally used as a reporter or tag for the visualization of genes as intracellular prove, it presents improved brightness and photostability over similar photoproteins.
How can this part be used
In AlgaGenix, mCherry sequence has been used for MoClo assembly as a B3-B5 piece to create a reporter cassette that can be adapted for position 6 in Level Ms.
Source of this part
Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018).
Design considerations
MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan. Sequence contains an intron (CrRbcS2 intron 1) for an intron-mediated enhancement (IME).
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 349
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 349
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 349
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 349
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Team UVU-Utah-2, 2024
We obtained this part in the Chlamydomonas MoClo kit as described in Crozet et al. (2018). We utilized and characterized this part in composite part(s) K5078005, K5078006. Please check out the documentation for that composite part for more details!