Difference between revisions of "Part:BBa I13600:Experience"
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The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. | The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. | ||
| − | Expression can be visualised very nicely under a flurescent microscope. | + | Expression can be visualised very nicely under a flurescent microscope. Even without having ideal filter. We used GFP filter for the picture below. |
| + | [[Image:2 I13600 flu60aberdeen2009.jpg|center|400 px]] | ||
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Revision as of 17:10, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I13600
- Grew up this Biobrick on a plate just to test its flourescence compared to the other constitutive flourescent proteins in the Registry as of July 2006. This has one of the weakest flourescent outputs. --Smelissali 19:39, 3 July 2006 (EDT)
User Reviews
UNIQ4b8cba5856ee3afb-partinfo-00000000-QINU
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Smelissali |
Brick works, but the cyan flourescent protein is definitely not the brightest reporter you could use, and thus not my first choice. --Smelissali 19:38, 3 July 2006 (EDT) |
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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Aberdeen_Scotland 2009 |
The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. Expression can be visualised very nicely under a flurescent microscope. Even without having ideal filter. We used GFP filter for the picture below. [[Image:2 I13600 flu60aberdeen2009.jpg|center|400 px]] |
UNIQ4b8cba5856ee3afb-partinfo-00000005-QINU