Difference between revisions of "Part:BBa K5115009"

Revision as of 15:02, 24 September 2024

hypB

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115018(ribozyme+RBS+hypB+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 336
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 25
Illegal NgoMIV site found at 871
Illegal AgeI site found at 823
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115018 BBa_K5115018(ribozyme+RBS+hypB+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115018 BBa_K5115018(ribozyme+RBS+hypB+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.
 <!-- -->