Difference between revisions of "Part:BBa K5115010"
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===Usage and Biology===
 
===Usage and Biology===
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It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts[https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)] and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.
+
It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)] and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.
  
  

Revision as of 14:51, 24 September 2024


hypF

contributed by Fudan iGEM 2023

Introduction

The hypF is a subunit of the hox and hyp operonBBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115019(ribozyme+RBS+hypF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module) and BBa_K5115067(mineral, F module), which are used for further experiments.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 424
    Illegal XhoI site found at 2263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 29
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 274
    Illegal NgoMIV site found at 635
    Illegal NgoMIV site found at 2035
    Illegal NgoMIV site found at 2435
  • 1000
    COMPATIBLE WITH RFC[1000]


References