Difference between revisions of "Part:BBa K5115009"
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__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | The hypB is a subunit of the hox and hyp operon[https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115018 BBa_K5115018(ribozyme+RBS+hypB+stem-loop)]. And then it will eventually make up parts like [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)], which are used for further experiments. | ||
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Revision as of 14:37, 24 September 2024
hypB
Introduction
The hypB is a subunit of the hox and hyp operonBBa_K5115020.
Usage and Biology
It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115018(ribozyme+RBS+hypB+stem-loop). And then it will eventually make up parts like BBa_K5115066(mineral, U module), which are used for further experiments.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 336
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 25
Illegal NgoMIV site found at 871
Illegal AgeI site found at 823 - 1000COMPATIBLE WITH RFC[1000]