Difference between revisions of "Part:BBa K257023:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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we use the BBa_J61002 to have the pFec link to RFP
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We use the BBa_J61002 to test Fec promoter. Both parts digested by XbaI and PstI and ligated.
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+
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+
  
 
===Source===
 
===Source===

Latest revision as of 16:56, 21 October 2009

fec promoter + RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2919
    Illegal XbaI site found at 2934
    Illegal PstI site found at 877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2919
    Illegal PstI site found at 877
    Illegal NotI site found at 870
    Illegal NotI site found at 2925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2919
    Illegal XbaI site found at 2934
    Illegal PstI site found at 877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2919
    Illegal XbaI site found at 2934
    Illegal PstI site found at 877
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1958


Design Notes

We use the BBa_J61002 to test Fec promoter. Both parts digested by XbaI and PstI and ligated.

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References