Difference between revisions of "Part:BBa K228001"

 
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__NOTOC__
 
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<partinfo>BBa_K228001 short</partinfo>
 
<partinfo>BBa_K228001 short</partinfo>
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This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.  
 
This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.  
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'''Reference'''
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Anderson JC, Voigt CA, and Arkin AP. Environmental signal integration by a modular AND gate. Molecular Systems Biology. 2007, 3:133
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:56, 21 October 2009

SupD-tRNA

The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated.

This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.

Reference Anderson JC, Voigt CA, and Arkin AP. Environmental signal integration by a modular AND gate. Molecular Systems Biology. 2007, 3:133

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 33
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36