Difference between revisions of "Part:BBa K195619"
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This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein. | This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein. | ||
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+ | == Experiment Data == | ||
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+ | [[Image:Chart (Lr2tEr7t) without line.png]] | ||
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Latest revision as of 16:56, 21 October 2009
pLacI with activator gene CII and pRE with GFP generator
This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.
Experiment Data
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1383