Difference between revisions of "Part:BBa K5374022:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K5374022 short</partinfo>
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<partinfo>BBa_K5374022 SequenceAndFeatures</partinfo>
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===Design Notes===
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Sequence was optimized to maintain COMP’s collagen-binding properties while preserving EGFP’s fluorescence for effective functional analysis.
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===Source===
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Derived from COMP gene, with EGFP from BBa_K1911005.

Latest revision as of 11:52, 24 September 2024


COMP-EGFP (Cartilage Oligomeric Matrix Protein). A protein that binds and stabilizes collagen struct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1306
    Illegal PstI site found at 133
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 133
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1949


Design Notes

Sequence was optimized to maintain COMP’s collagen-binding properties while preserving EGFP’s fluorescence for effective functional analysis.


Source

Derived from COMP gene, with EGFP from BBa_K1911005.