Difference between revisions of "Part:BBa K5115003"
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__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| − | The hoxU is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. | + | The hoxU is a subunit of the hox and hyp operon[https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012]in case that it can be expressed smoothly in a polucostronic sequences. | + | It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme system[https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012]in case that it can be expressed smoothly in a polucostronic sequences. |
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Revision as of 11:14, 24 September 2024
hoxU, without stop codon
Introduction
The hoxU is a subunit of the hox and hyp operonBBa_K5115020.
Usage and Biology
It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme systemBBa_K5115012in case that it can be expressed smoothly in a polucostronic sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 410
Illegal BglII site found at 488
Illegal XhoI site found at 418
Illegal XhoI site found at 610 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 371
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 192
Illegal BsaI site found at 354
Illegal SapI.rc site found at 303