Difference between revisions of "Part:BBa K5205000"
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<figcaption><center>Figure 1. A. Predicted 3D structure of OsMTI-1b (Jumper et al., 2021; Varadi et al., 2024); B. General structure of metallothionein, with four and three metal ions coordinated in the α- and β-domains, respectively (Klaassen et al., 1999). </center></figcaption> | <figcaption><center>Figure 1. A. Predicted 3D structure of OsMTI-1b (Jumper et al., 2021; Varadi et al., 2024); B. General structure of metallothionein, with four and three metal ions coordinated in the α- and β-domains, respectively (Klaassen et al., 1999). </center></figcaption> |
Revision as of 02:43, 24 September 2024
OsMTI-1b, metallothionein from Oryza sativa subsp. japonica
OsMTI-1b (also noted as OsMT1) encodes for a metallothionein (MT) in rice (Oryza sativa subsp. japonica) (Buell et al., 2005). As a metallothionein, OsMTI-1b binds to metal ions, involving metal ion transport, antioxidant defense, and maintaining metal ion balance in rice (Ruttkay-Nedecky et al., 2013; Thirumoorthy et al., 2007). This part is codon-optimized for E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Buell, C. R., Yuan, Q., Ouyang, S., Liu, J., Zhu, W., Wang, A., Maiti, R., Haas, B., Wortman, J., Pertea, M., Jones, K. M., Kim, M., Overton, L., Tsitrin, T., Fadrosh, D., Bera, J., Weaver, B., Jin, S., Johri, S., . . . Jackson, S. (2005). Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species. Genome Res, 15(9), 1284-1291. https://doi.org/10.1101/gr.3869505
Klaassen, C. D., Liu, J., & Choudhuri, S. (1999). Metallothionein: an intracellular protein to protect against cadmium toxicity. Annual review of pharmacology and toxicology, 39, 267-294.
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., Bridgland, A., Meyer, C., Kohl, S. A. A., Ballard, A. J., Cowie, A., Romera-Paredes, B., Nikolov, S., Jain, R., Adler, J., Back, T., Petersen, S., Reiman, D., Clancy, E., Zielinski, M., Steinegger, M., Pacholska, M., Berghammer, T., Bodenstein, S., Silver, D., Vinyals, O., Senior, A. W., Kavukcuoglu, K., Kohli, P., & Hassabis, D. (2021). Highly accurate protein structure prediction with AlphaFold. Nature, 596(7873), 583–589. https://doi.org/10.1038/s41586-021-03819-2
Ruttkay-Nedecky, B., Nejdl, L., Gumulec, J., Zitka, O., Masarik, M., Eckschlager, T., Stiborova, M., Adam, V., & Kizek, R. (2013). The role of metallothionein in oxidative stress. Int J Mol Sci, 14(3), 6044-6066. https://doi.org/10.3390/ijms14036044
Thirumoorthy, N., Manisenthil Kumar, K. T., Shyam Sundar, A., Panayappan, L., & Chatterjee, M. (2007). Metallothionein: an overview. World J Gastroenterol, 13(7), 993-996. https://doi.org/10.3748/wjg.v13.i7.993
Varadi, M., Bertoni, D., Magana, P., Paramval, U., Pidruchna, I., Radhakrishnan, M., Tsenkov, M., Nair, S., Mirdita, M., Yeo, J., Kovalevskiy, O., Tunyasuvunakool, K., Laydon, A., Žídek, A., Tomlinson, H., Hariharan, D., Abrahamson, J., Green, T., Jumper, J., Birney, E., Steinegger, M., Hassabis, D., & Velankar, S. (2024). AlphaFold Protein Structure Database in 2024: Providing structure coverage for over 214 million protein sequences. Nucleic Acids Research. https://doi.org/10.1093/nar/gkad117