Difference between revisions of "Part:BBa K5115026:Design"

(→‎Design Notes)
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===References===
 
===References===
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[1]Li T, Jiang Q, Huang J, et al. Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020;11(1):5448. Published 2020 Oct 28. doi:10.1038/s41467-020-19280-0

Revision as of 02:08, 24 September 2024


csoS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


Design Notes

Source

The part is originally identified in a species of cyanobacteria, Halothiobacillus neapolitanus.

In our experiment, the CsoS2 part was obtained from a plasmid sourced from the Addgene repository (plasmid #140856).

References

[1]Li T, Jiang Q, Huang J, et al. Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020;11(1):5448. Published 2020 Oct 28. doi:10.1038/s41467-020-19280-0