Difference between revisions of "Part:BBa K5115026"

(Introduction)
Line 6: Line 6:
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
 +
CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium ''Halothiobacillus neapolitanus'', CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in ''E. coli''.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:10, 24 September 2024


csoS2

contributed by Fudan iGEM 2023

Introduction

CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium Halothiobacillus neapolitanus, CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in E. coli.

Usage and Biology

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References