Difference between revisions of "Part:BBa K5237004"
Line 1: Line 1:
−
 
+
<!--################################-->
 +
<!--Provided by the iGEM registry main page when editing a part, DO NOT copy information from template-->
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K5237000 short</partinfo>
+
<partinfo>BBa_K5237000</partinfo>
 +
<!--################################-->
 +
 
 +
<!--Add changes below--->
 
<html>
 
<html>
−
  <style>
+
<style>
−
    p {
+
  p {
−
      text-align: justify;
+
    text-align: justify;
−
      margin-right: 25px;
+
    margin-right: 25px;
−
      font-style: normal;
+
    font-style: normal;
−
    }
+
  }
−
    section {
+
 
−
      margin-left: 25px;
+
  section {
−
      margin-right: 25px;
+
    margin-left: 25px;
−
      margin-top: 25px;
+
    margin-right: 25px;
−
     }
+
    margin-top: 25px;
 +
  }
 +
 
 +
  .thumb {
 +
     width: 100%;
 +
  }
 +
 
 +
  table,
 +
  th,
 +
  td {
 +
    border: 0.5px solid black;
 +
    border-collapse: collapse;
 +
  }
 +
 
 +
  th,
 +
  td {
 +
    padding: 1.5px;
 +
  }
 +
</style>
  
−
    .thumb {
 
−
      width: 100%;
 
−
    }
 
−
  </style>
 
 
<body>
 
<body>
−
    <section id="1">
+
  <!-- Part summary -->
−
      <p>Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex
+
  <section id="1">
−
        senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis
+
    <h1>!!!Part Name!!!</h1>
−
        natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod
+
    <p>Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex
−
        morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat
+
      senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis
−
        nascetur.</p>
+
      natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod
−
      <p>&nbsp;</p>
+
      morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat
−
    </section>
+
      nascetur.</p>
 +
    <p>&nbsp;</p>
 +
  </section>
 
   <div id="toc" class="toc">
 
   <div id="toc" class="toc">
 
     <div id="toctitle">
 
     <div id="toctitle">
Line 50: Line 70:
 
     </ul>
 
     </ul>
 
   </div>
 
   </div>
 +
  <section id="2">
 +
    <font size="5"><b>The PICasSO Toolbox </b> </font>
 +
 +
    <p>The PICasSO toolbox provides a comprehensive platform for engineering, optimizing and testing new systems for
 +
      spatial DNA organization. With PiCasSo, you can efficiently design and test new systems for a wide range of
 +
      synthetic biology applications.
 +
      <br>
 +
      Our parts collection includes:
 +
        <table style="width: 55%;">
 +
          <td colspan="2" align="left"><b>DNA-binding proteins: </b>
 +
            The building blocks for engineering of custom staples for DNA-DNA interactions with a modular system ensuring
 +
            easy assembly.</td>
 +
          <tbody>
 +
            <tr>
 +
            <tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926000" target="_blank">BBa_K5237000</a>
 +
              </td>
 +
              <td>tetR-Oct1 Simple Staple
 +
              </td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926001" target="_blank">BBa_K5237001</a>
 +
              </td>
 +
              <td>fgRNA Entryvector MbCas12a-SpCas9
 +
              </td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926002" target="_blank">BBa_K5237002</a>
 +
              </td>
 +
              <td>dMbCas12a
 +
              </td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926003" target="_blank">BBa_K5237003</a>
 +
              </td>
 +
              <td>dSpCas9
 +
              </td>
 +
            </tr>
 +
            <tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926004" target="_blank">BBa_K5237004</a>
 +
              </td>
 +
              <td>dMbCas12a-dSpCas9 "dFusionCas"
 +
              </td>
 +
            </tr>
 +
          </tbody>
 +
          <td colspan="2" align="left"><b>Functional elements: </b>
 +
            Protease cleavable peptide linkers and inteins are used to control and modify staples for further optimization
 +
            for custom applications.</td>
 +
            <tbody>
 +
              <tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K2926005" target="_blank">BBa_K5237005</a>
 +
                </td>
 +
                <td>fgRNA Processing Casette
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K2926006" target="_blank">BBa_K5237006</a>
 +
                </td>
 +
                <td>Cathepsin B Cleavable Linker
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K2926007" target="_blank">BBa_K5237007</a>
 +
                </td>
 +
                <td>Cathepsin Expression Casette
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K2926008" target="_blank">BBa_K5237008</a>
 +
                </td>
 +
                <td>Caged NpuN Intein
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K2926009" target="_blank">BBa_K5237009</a>
 +
                </td>
 +
                <td>Caged NpuC Intein
 +
                </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926010" target="_blank">BBa_K5237010</a>
 +
              </td>
 +
              <td>Conjugation Origin of Transfer (oriT)
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926011" target="_blank">BBa_K5237011</a>
 +
              </td>
 +
              <td>Intimin Anti-EGFR Nanobody
 +
              </td>
 +
              </tr>
 +
            </tbody>
 +
          <td colspan="2" align="left"><b>Functional elements: </b>
 +
            Protease cleavable peptide linkers and inteins are used to control and modify staples for further optimization
 +
            for custom applications.</td>
 +
            <tbody>
 +
              <tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K29260012" target="_blank">BBa_K5237012</a>
 +
                </td>
 +
                <td>GCN4 Leucine Zipper
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K29260013" target="_blank">BBa_K5237013</a>
 +
                </td>
 +
                <td>rGCN4 Reverse Leucine Zipper
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K29260014" target="_blank">BBa_K5237014</a>
 +
                </td>
 +
                <td>mNeonGreen-Oct1-DBD Fusion Protein
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K29260015" target="_blank">BBa_K5237015</a>
 +
                </td>
 +
                <td>TetR-mScarlet-I Fusion Protein
 +
                </td>
 +
              </tr>
 +
              <tr>
 +
                <td><a href="https://parts.igem.org/Part:BBa_K29260016" target="_blank">BBa_K5237016</a>
 +
                </td>
 +
                <td>Oct1 Binding Casette
 +
                </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926017" target="_blank">BBa_K5237017</a>
 +
              </td>
 +
              <td>Tet Response Element Binding Casette
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926018" target="_blank">BBa_K5237018</a>
 +
              </td>
 +
              <td>SV40-Gal4-GFLG-VP64 Fusion Protein
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926019" target="_blank">BBa_K5237019</a>
 +
              </td>
 +
              <td>SV40-Gal4-VP64 Fusion Protein
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926020" target="_blank">BBa_K5237020</a>
 +
              </td>
 +
              <td>UAS - mCherry Expression Casette
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926021" target="_blank">BBa_K5237021</a>
 +
              </td>
 +
              <td>Gal4-VP64 Fusion Protein
 +
              </td>
 +
              </tr>
 +
              <td><a href="https://parts.igem.org/Part:BBa_K2926022" target="_blank">BBa_K5237022</a>
 +
              </td>
 +
              <td> 5x UAS binding Firefly-Luciferase Minimal Expression Casette</td>
 +
            </tbody>
 +
          </table>
 +
    </p>
 +
  </section>
 
   <section id="1">
 
   <section id="1">
 
     <h1>1. Sequence overview</h1>
 
     <h1>1. Sequence overview</h1>
Line 57: Line 235:
 
</html>
 
</html>
  
 +
<!--################################-->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5237000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5237000 SequenceAndFeatures</partinfo>
 +
<!--################################-->
 +
 +
<html>
 +
  
−
<html> 
 
 
<body>
 
<body>
 
   <section id="2">
 
   <section id="2">
 
     <h1>2. Usage and Biology</h1>
 
     <h1>2. Usage and Biology</h1>
 
     <div class="thumb">
 
     <div class="thumb">
−
       <div class="thumbinner" style="width:452px;"><a href="placeholder"
+
       <div class="thumbinner" style="width:452px;"><a href="placeholder" class="image"><img alt=""
−
          class="image"><img alt="" src="https://static.igem.wiki/teams/5237/picasso-iconlr-transp.svg" width="450"
+
            src="https://static.igem.wiki/teams/5237/picasso-iconlr-transp.svg" width="450" height="313"
−
            height="313" class="thumbimage"></a>
+
            class="thumbimage"></a>
 
         <div class="thumbcaption">
 
         <div class="thumbcaption">
 
           <i><b>Figure 1</b></i><br>
 
           <i><b>Figure 1</b></i><br>
Line 98: Line 280:
 
   <section id="5">
 
   <section id="5">
 
     <h1>5. References</h1>
 
     <h1>5. References</h1>
 +
    <p>Wu, W., Zhang, L., Yao, L., Tan, X., Liu, X., & Lu, X. (2015). Genetically assembled fluorescent biosensor
 +
      for in situ detection of bio-synthesized alkanes. Scientific reports, 5, 10907. <a
 +
        href="https://doi.org/10.1038/srep10907" target="_blank">https://doi.org/10.1038/srep10907</a></p>
 
   </section>
 
   </section>
 
</body>
 
</body>
  
 
</html>
 
</html>
−
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
−
<partinfo>BBa_K5237000 parameters</partinfo>
 
−
<!-- -->
 

Revision as of 22:59, 23 September 2024


BBa_K5237000

!!!Part Name!!!

Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat nascetur.

 

The PICasSO Toolbox

The PICasSO toolbox provides a comprehensive platform for engineering, optimizing and testing new systems for spatial DNA organization. With PiCasSo, you can efficiently design and test new systems for a wide range of synthetic biology applications.
Our parts collection includes:

DNA-binding proteins: The building blocks for engineering of custom staples for DNA-DNA interactions with a modular system ensuring easy assembly.
BBa_K5237000 tetR-Oct1 Simple Staple
BBa_K5237001 fgRNA Entryvector MbCas12a-SpCas9
BBa_K5237002 dMbCas12a
BBa_K5237003 dSpCas9
BBa_K5237004 dMbCas12a-dSpCas9 "dFusionCas"
Functional elements: Protease cleavable peptide linkers and inteins are used to control and modify staples for further optimization for custom applications.
BBa_K5237005 fgRNA Processing Casette
BBa_K5237006 Cathepsin B Cleavable Linker
BBa_K5237007 Cathepsin Expression Casette
BBa_K5237008 Caged NpuN Intein
BBa_K5237009 Caged NpuC Intein
BBa_K5237010 Conjugation Origin of Transfer (oriT)
BBa_K5237011 Intimin Anti-EGFR Nanobody
Functional elements: Protease cleavable peptide linkers and inteins are used to control and modify staples for further optimization for custom applications.
BBa_K5237012 GCN4 Leucine Zipper
BBa_K5237013 rGCN4 Reverse Leucine Zipper
BBa_K5237014 mNeonGreen-Oct1-DBD Fusion Protein
BBa_K5237015 TetR-mScarlet-I Fusion Protein
BBa_K5237016 Oct1 Binding Casette
BBa_K5237017 Tet Response Element Binding Casette
BBa_K5237018 SV40-Gal4-GFLG-VP64 Fusion Protein
BBa_K5237019 SV40-Gal4-VP64 Fusion Protein
BBa_K5237020 UAS - mCherry Expression Casette
BBa_K5237021 Gal4-VP64 Fusion Protein
BBa_K5237022 5x UAS binding Firefly-Luciferase Minimal Expression Casette

1. Sequence overview

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 571
    Illegal SapI site found at 662
    Illegal SapI.rc site found at 280

2. Usage and Biology

Figure 1
Lorem Ipsum

Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat nascetur.

3. Assembly and part evolution

Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat nascetur.

4. Results

Lorem ipsum odor amet, consectetuer adipiscing elit. Mi volutpat nunc himenaeos malesuada, fermentum metus ex senectus proin. Primis natoque adipiscing ultricies volutpat dictum varius auctor molestie euismod. Platea primis natoque vivamus himenaeos sagittis habitant mauris pulvinar. Scelerisque morbi praesent turpis convallis euismod morbi. Eget est iaculis morbi molestie lacus ornare mollis massa ut. Turpis nam odio quis erat nunc placerat nascetur.

5. References

Wu, W., Zhang, L., Yao, L., Tan, X., Liu, X., & Lu, X. (2015). Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes. Scientific reports, 5, 10907. https://doi.org/10.1038/srep10907