Difference between revisions of "Part:BBa K257019"

Latest revision as of 16:33, 21 October 2009

FecA mutated - constitutive activity of the fec operon

The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.

In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.

We designed FecA to have a constitutive activity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1290
Illegal BglII site found at 2203
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 89

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 __NOTOC__
 <partinfo>BBa_K257019 short</partinfo>
-FecA is a protein that binds the ferric citrate and enabled a transduction pathway that leads to the expression of the fec operon.
-We designed it to have a constitutive activity.
+The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.
+In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.
+We designed FecA to have a constitutive activity.
 <!-- Add more about the biology of this part here