Difference between revisions of "Part:BBa K257019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The original sequence contained 1 Pst1 site and required | + | The original sequence contained 1 Pst1 site and required one additional mutation to obtain its constitutive activity. |
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===Source=== | ===Source=== | ||
Latest revision as of 16:18, 21 October 2009
FecA mutated - constitutive activity of the fec operon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1290
Illegal BglII site found at 2203 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 89
Design Notes
The original sequence contained 1 Pst1 site and required one additional mutation to obtain its constitutive activity.
Source
K12 genomic DNA