Difference between revisions of "Part:BBa K191004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | * | + | * Trp promoter was added to plasmid containing part <partinfo>BBa_I13507</partinfo> from iGEM kit using [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow Klenow protocol]. |
| − | + | * Trp promoter as PCR products in Klenow protocol was cut with E and S to be ligated into plasmid with RFP gene (<partinfo>BBa_I13507</partinfo>), which was also cut with E and S. | |
===Source=== | ===Source=== | ||
Revision as of 16:06, 21 October 2009
TRP promoter - RBS - RFP - Term
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 43
Illegal SpeI site found at 51
Illegal AgeI site found at 666
Illegal AgeI site found at 778 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Trp promoter was added to plasmid containing part BBa_I13507 from iGEM kit using [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow Klenow protocol].
- Trp promoter as PCR products in Klenow protocol was cut with E and S to be ligated into plasmid with RFP gene (BBa_I13507), which was also cut with E and S.