Difference between revisions of "Part:BBa K257002"
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ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins. | ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins. | ||
| − | + | PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits. | |
| − | + | ||
| − | PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits. | + | |
*ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs | *ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs | ||
*We can fuse GFP to the C or N term of Cly A, to track OMVs easily. | *We can fuse GFP to the C or N term of Cly A, to track OMVs easily. | ||
*ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host. | *ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host. | ||
*Cly A confers vesicle binding to and invasion of host cells. | *Cly A confers vesicle binding to and invasion of host cells. | ||
| − | *ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins. | + | *ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.<!-- Add more about the biology of this part here |
| + | ===Usage and Biology=== | ||
| + | |||
Revision as of 16:05, 21 October 2009
ClyA_Fusion_C-Term
ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.
PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.
- ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
- We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
- ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
- Cly A confers vesicle binding to and invasion of host cells.
- ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 85
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]