Difference between revisions of "Part:BBa K191004:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K191004 short</partinfo>
 
<partinfo>BBa_K191004 short</partinfo>
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===Design Notes===
 
===Design Notes===
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TRP operon was added by PCR.  
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* TRP promoter was added to plasmid containing part <partinfo>BBa_I13507</partinfo> from iGEM kit by [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow Klenow protocol].
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+
  
  
 
===Source===
 
===Source===
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<partinfo>BBa_K191007</partinfo>, <partinfo>BBa_I13507</partinfo>
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See subpart's reference
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===References===
 
===References===

Revision as of 16:02, 21 October 2009

TRP promoter - RBS - RFP - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
    Illegal AgeI site found at 666
    Illegal AgeI site found at 778
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • TRP promoter was added to plasmid containing part BBa_I13507 from iGEM kit by [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow Klenow protocol].


Source

BBa_K191007, BBa_I13507

References