Difference between revisions of "Part:BBa K5208010"

 
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This is an expression cassette consisting of a strong inducible promoter Pgrac, a secretion signal peptide SPamyQ, the AHL lactonase AiiA, and a terminator.
 
This is an expression cassette consisting of a strong inducible promoter Pgrac, a secretion signal peptide SPamyQ, the AHL lactonase AiiA, and a terminator.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K5208010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5208010 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K5208010 parameters</partinfo>
 
<partinfo>BBa_K5208010 parameters</partinfo>
 
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===Usage and Biology===
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Aeromonas hydrophila is an aquatic pathogen that poses a significant threat to the aquaculture industry. Its virulence is primarily regulated by quorum sensing, a mechanism triggered by acyl-homoserine lactones (AHLs), particularly N-butanoyl-L-homoserine lactone (C4-HSL) (Coquant et al., 2020; Hlordzi et al., 2020).
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In this expression cassette, Pgrac [https://parts.igem.org/Part:BBa_K1628202] is a strong promoter, and SPamyQ (BBa_K1074014) is a signal peptide that ensures the efficient secretion of the AHL lactonase. AiiA (BBa_K5208000) is an enzyme that catalyzes the hydrolysis of AHL. By expressing AiiA in the probiotic Bacillus subtilis and introducing the engineered bacteria into fish ponds, quorum sensing in A. hydrophila can be disrupted, reducing its virulence and safeguarding aquatic animals. Finally, BBa_K4934022 is the terminator.

Revision as of 05:14, 23 September 2024


Pgrac-SPamyQ-AiiA-Term

This is an expression cassette consisting of a strong inducible promoter Pgrac, a secretion signal peptide SPamyQ, the AHL lactonase AiiA, and a terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

Aeromonas hydrophila is an aquatic pathogen that poses a significant threat to the aquaculture industry. Its virulence is primarily regulated by quorum sensing, a mechanism triggered by acyl-homoserine lactones (AHLs), particularly N-butanoyl-L-homoserine lactone (C4-HSL) (Coquant et al., 2020; Hlordzi et al., 2020).

In this expression cassette, Pgrac [1] is a strong promoter, and SPamyQ (BBa_K1074014) is a signal peptide that ensures the efficient secretion of the AHL lactonase. AiiA (BBa_K5208000) is an enzyme that catalyzes the hydrolysis of AHL. By expressing AiiA in the probiotic Bacillus subtilis and introducing the engineered bacteria into fish ponds, quorum sensing in A. hydrophila can be disrupted, reducing its virulence and safeguarding aquatic animals. Finally, BBa_K4934022 is the terminator.