Difference between revisions of "Part:BBa K257007:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
For fusion of proteins to the N-terminal extremity of ClyA using the silver fusion standard RFC23
+
For fusion of proteins to the N-terminal extremity of ClyA using the silver fusion standard RFC23. We also need a poly gly linker at the N-terminal extremity.
 +
 
 +
This part is inserted in PSB1A3
  
 
===Source===
 
===Source===

Latest revision as of 15:56, 21 October 2009

ClyA_Fusion_N-Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For fusion of proteins to the N-terminal extremity of ClyA using the silver fusion standard RFC23. We also need a poly gly linker at the N-terminal extremity.

This part is inserted in PSB1A3

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References