Difference between revisions of "Part:BBa K5205001"
Line 3: Line 3:
 
<partinfo>BBa_K5205001 short</partinfo>
 
<partinfo>BBa_K5205001 short</partinfo>
  
−
YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012).
+
The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).
−
 
+
−
<html>
+
−
<body>
+
−
<figure>
+
−
<div class = "center">
+
−
<center><img src = "https://static.igem.wiki/teams/5208/parts/00-1.png" style = "width:600px"></center>
+
−
</div>
+
−
<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by YtnP. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000). </center></figcaption>
+
−
</figure>
+
−
</body>
+
−
</html>
+
  
  
Line 31: Line 20:
 
<!-- Add more about the biology of this part here  -->
 
<!-- Add more about the biology of this part here  -->
 
===Usage and Biology===
 
===Usage and Biology===
−
YtnP degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of YtnP in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
+
The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) (BBa_K5205002) to form the Non-OmpA system (BBa_K5205003). This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).
  
−
<html>
 
−
<body>
 
−
<figure>
 
−
<div class = "center">
 
−
<center><img src = "https://static.igem.wiki/teams/5208/parts/01-2.png" style = "width:300px"></center>
 
−
</div>
 
−
<figcaption><center>Figure 2. Gel electrophoresis of PCR product of YtnP. </center></figcaption>
 
−
</figure>
 
−
</body>
 
−
</html>
 
  
 
==References==
 
==References==
−
their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
+
Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0
−
 
+
−
Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
+
−
 
+
−
Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
+
−
 
+
−
Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
+
−
 
+
−
Schneider, J., Yepes, A., Garcia-Betancur, J. C., Westedt, I., Mielich, B., & López, D. (2012). Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus. Appl Environ Microbiol, 78(2), 599-603. https://doi.org/10.1128/aem.06992-11
+

Revision as of 04:48, 23 September 2024


Non, signal-like sequence

The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) (BBa_K5205002) to form the Non-OmpA system (BBa_K5205003). This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).


References

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0