Difference between revisions of "Part:BBa K5208003"
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AiiM encodes for an N-acyl homoserine lactonase in M. testaceum StLB037. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Wang et al., 2010). | AiiM encodes for an N-acyl homoserine lactonase in M. testaceum StLB037. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Wang et al., 2010). | ||
− | < | + | <html> |
− | === | + | <body> |
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.wiki/teams/5208/parts/00-1.png" style = "width:600px"></center> | ||
+ | </div> | ||
+ | <figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AiiM. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).</center></figcaption> | ||
+ | </figure> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
+ | ===Sequence and Features=== | ||
<partinfo>BBa_K5208003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5208003 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K5208003 parameters</partinfo> | <partinfo>BBa_K5208003 parameters</partinfo> | ||
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+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here--> | ||
+ | ===Usage and Biology=== | ||
+ | AiiM degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiM in the probiotic Bacillus subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals. | ||
+ | |||
+ | <html> | ||
+ | <body> | ||
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.wiki/teams/5208/parts/03-2.png" style = "width:300px"></center> | ||
+ | </div> | ||
+ | <figcaption><center>Figure 2. Gel electrophoresis of PCR product of AiiM. </center></figcaption> | ||
+ | </figure> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | ==References== | ||
+ | Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477 | ||
+ | |||
+ | Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827 | ||
+ | |||
+ | Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526 | ||
+ | |||
+ | Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503 | ||
+ | |||
+ | Wang, W. Z., Morohoshi, T., Ikenoya, M., Someya, N., & Ikeda, T. (2010). AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol, 76(8), 2524-2530. https://doi.org/10.1128/AEM.02738-09 |
Revision as of 04:18, 23 September 2024
AiiM, N-acyl homoserine lactonase from Microbacterium testaceum StLB037
AiiM encodes for an N-acyl homoserine lactonase in M. testaceum StLB037. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Wang et al., 2010).
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 516
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 81
Illegal BamHI site found at 575
Illegal XhoI site found at 526 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 482
Illegal BsaI site found at 610
Illegal SapI site found at 297
Usage and Biology
AiiM degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiM in the probiotic Bacillus subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
References
Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
Wang, W. Z., Morohoshi, T., Ikenoya, M., Someya, N., & Ikeda, T. (2010). AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol, 76(8), 2524-2530. https://doi.org/10.1128/AEM.02738-09