Difference between revisions of "Part:BBa K5208002"

 
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AttM encodes for an N-acyl homoserine lactonase in A. tumefaciens C58. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Carlier et al., 2003).
 
AttM encodes for an N-acyl homoserine lactonase in A. tumefaciens C58. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Carlier et al., 2003).
  
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===Usage and Biology===
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<center><img src = "https://static.igem.wiki/teams/5208/parts/00-1.png" style = "width:600px"></center>
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<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AttM. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000). </center></figcaption>
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K5208002 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K5208002 parameters</partinfo>
 
<partinfo>BBa_K5208002 parameters</partinfo>
 
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===Usage and Biology===
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AttM degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AttM in the probiotic Bacillus subtilis may reduce the virulence of A. hydrophila through quorum quenching and save aquatic animals.
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<center><img src = "https://static.igem.wiki/teams/5208/parts/02-2.png" style = "width:300px"></center>
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<figcaption><center>Figure 2. Gel electrophoresis of PCR product of AttM. </center></figcaption>
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==References==
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Carlier, A., Uroz, S., Smadja, B., Fray, R., Latour, X., Dessaux, Y., & Faure, D. (2003). The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity. Appl Environ Microbiol, 69(8), 4989-4993. https://doi.org/10.1128/aem.69.8.4989-4993.2003
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Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
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Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
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Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
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Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503

Revision as of 04:07, 23 September 2024


AttM, N-acyl homoserine lactonase from Agrobacterium tumefaciens C58

AttM encodes for an N-acyl homoserine lactonase in A. tumefaciens C58. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Carlier et al., 2003).

Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AttM. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

AttM degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AttM in the probiotic Bacillus subtilis may reduce the virulence of A. hydrophila through quorum quenching and save aquatic animals.

Figure 2. Gel electrophoresis of PCR product of AttM.

References

Carlier, A., Uroz, S., Smadja, B., Fray, R., Latour, X., Dessaux, Y., & Faure, D. (2003). The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity. Appl Environ Microbiol, 69(8), 4989-4993. https://doi.org/10.1128/aem.69.8.4989-4993.2003

Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477

Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827

Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526

Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503