Difference between revisions of "Part:BBa K5205001"

 
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<partinfo>BBa_K5205001 short</partinfo>
 
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YtnP, N-acyl homoserine lactonase from Bacillus subtilis WB600
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YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012).
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<center><img src = "https://static.igem.wiki/teams/5208/parts/00-1.png" style = "width:600px"></center>
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<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by YtnP. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000). </center></figcaption>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'></span>
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===Sequence and Features===
 
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<partinfo>BBa_K5205001 parameters</partinfo>
 
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<!-- Add more about the biology of this part here  -->
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===Usage and Biology===
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YtnP degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of YtnP in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
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<center><img src = "https://static.igem.wiki/teams/5208/parts/01-2.png" style = "width:300px"></center>
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<figcaption><center>Figure 2. Gel electrophoresis of PCR product of AiiA. </center></figcaption>
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==References==
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their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
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Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
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Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
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Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
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Schneider, J., Yepes, A., Garcia-Betancur, J. C., Westedt, I., Mielich, B., & López, D. (2012). Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus. Appl Environ Microbiol, 78(2), 599-603. https://doi.org/10.1128/aem.06992-11

Revision as of 04:00, 23 September 2024


Non, signal-like sequence

YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012).

Figure 1. Degradation of N-acyl homoserine lactone (AHL) by YtnP. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

YtnP degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of YtnP in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.

Figure 2. Gel electrophoresis of PCR product of AiiA.

References

their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477

Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827

Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526

Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503

Schneider, J., Yepes, A., Garcia-Betancur, J. C., Westedt, I., Mielich, B., & López, D. (2012). Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus. Appl Environ Microbiol, 78(2), 599-603. https://doi.org/10.1128/aem.06992-11