Difference between revisions of "Part:BBa K5299121"

 
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<partinfo>BBa_K5299121 short</partinfo>
 
<partinfo>BBa_K5299121 short</partinfo>
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<h1>Biology</h1>
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THI20 (phosphomethylpyrimidine kinase) (XM_009655087.1) has a role in V. dahliae's thiamine biosynthesis pathway.<br>
  
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THI20 (phosphomethylpyrimidine kinase) (XM_009655087.1) has a role in V. dahliae's thiamine biosynthesis pathway.
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Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1].
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<h1>Use</h1>
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Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-&#946;-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1].
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This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].<br>
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We designed this part to test if our bacteria could produce dsRNA targeting this gene.
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This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1]
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<h1>References</h1>
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We used this gene as a target for dsRNA molecules in order to impair fungal growth.  
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[1] Qin, T., Hao, W., Sun, R., Li, Y., Wang, Y., Wei, C., … Wang, Q. (2020). Verticillium dahliae VdTHI20, involved in pyrimidine biosynthesis, is required for DNA repair functions and pathogenicity. International Journal of Molecular Sciences, 21(4), 1378. doi:10.3390/ijms21041378
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:52, 23 September 2024


THI20 protein (V. dahliae)

Biology

THI20 (phosphomethylpyrimidine kinase) (XM_009655087.1) has a role in V. dahliae's thiamine biosynthesis pathway.

Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1].

Use

This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].
We designed this part to test if our bacteria could produce dsRNA targeting this gene.

References

[1] Qin, T., Hao, W., Sun, R., Li, Y., Wang, Y., Wei, C., … Wang, Q. (2020). Verticillium dahliae VdTHI20, involved in pyrimidine biosynthesis, is required for DNA repair functions and pathogenicity. International Journal of Molecular Sciences, 21(4), 1378. doi:10.3390/ijms21041378 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]