Difference between revisions of "Part:BBa K5299119"

 
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<partinfo>BBa_K5299119 short</partinfo>
 
<partinfo>BBa_K5299119 short</partinfo>
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<h1>Biology</h1>
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VdRGS1 (MG583845.1) codes for a negative regulator of G protein signaling in V. dahliae and plays a role in many development stages of the fungus including spore germination, hyphal development, spore production, and microsclerotia formation [1]. <br>
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The RGS1 protein is part of the G signaling pathway. By assisting the Gα subunit hydrolyse GTP it turns off the G protein-coupled signaling pathways and stops downstream signals [2].
  
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VdRGS1 (MG583845.1) codes for a negative regulator of G protein signaling in V. dahliae and plays a role in many development stages of the fungus.
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<h1>Use</h1>
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The RGS1 protein is part of the G signaling pathway. By assisting the G&#945; subunits hydrolyse GTP it turns off the G protein-coupled signaling pathways and stops downstream signals [1].
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This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].
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In V. dahliae it plays a role in many developmental stages of the fungus, including spore germination, hyphal development, spore production, and microsclerotia formation [2].
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This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [2].
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We designed this part to test if our bacteria could produce dsRNA targeting this fungal protein.  
 
We designed this part to test if our bacteria could produce dsRNA targeting this fungal protein.  
  
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<h1>References</h1>
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[1] Xu, J., Wang, X., Li, Y., Zeng, J., Wang, G., Deng, C., & Guo, W. (2018). Host-induced gene silencing of a regulator of G protein signalling gene (VdRGS1) confers resistance to Verticillium wilt in cotton. Plant Biotechnology Journal, 16(9), 1629–1643. doi:10.1111/pbi.12900 <br>
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[2] Tuteja N. (2009). Signaling through G protein coupled receptors. Plant signaling & behavior, 4(10), 942–947. https://doi.org/10.4161/psb.4.10.9530
  
 
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<!-- Add more about the biology of this part here

Revision as of 00:27, 23 September 2024


RGS1 protein V. dahliae

Biology

VdRGS1 (MG583845.1) codes for a negative regulator of G protein signaling in V. dahliae and plays a role in many development stages of the fungus including spore germination, hyphal development, spore production, and microsclerotia formation [1].
The RGS1 protein is part of the G signaling pathway. By assisting the Gα subunit hydrolyse GTP it turns off the G protein-coupled signaling pathways and stops downstream signals [2].

Use

This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1]. We designed this part to test if our bacteria could produce dsRNA targeting this fungal protein.

References

[1] Xu, J., Wang, X., Li, Y., Zeng, J., Wang, G., Deng, C., & Guo, W. (2018). Host-induced gene silencing of a regulator of G protein signalling gene (VdRGS1) confers resistance to Verticillium wilt in cotton. Plant Biotechnology Journal, 16(9), 1629–1643. doi:10.1111/pbi.12900
[2] Tuteja N. (2009). Signaling through G protein coupled receptors. Plant signaling & behavior, 4(10), 942–947. https://doi.org/10.4161/psb.4.10.9530

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 98