Difference between revisions of "Part:BBa K5286001:Design"

 
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===References===
 
===References===
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Boothby, T. C., Tapia, H., Brozena, A. H., Piszkiewicz, S., Smith, A. E., Giovannini, I., Rebecchi, L., Pielak, G. J., Koshland, D., & Goldstein, B. (2017). Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation. Molecular Cell, 65(6), 975. https://doi.org/10.1016/j.molcel.2017.02.018

Revision as of 21:12, 22 September 2024


Cytosolic-abundant heat soluble protein 94063 (CAHS94063)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNA retrotrasncription and it has already been codon optimized for expression in E. coli.



Source

This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNAseq, and it has already been codon optimized for expression in E. coli.

References

Boothby, T. C., Tapia, H., Brozena, A. H., Piszkiewicz, S., Smith, A. E., Giovannini, I., Rebecchi, L., Pielak, G. J., Koshland, D., & Goldstein, B. (2017). Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation. Molecular Cell, 65(6), 975. https://doi.org/10.1016/j.molcel.2017.02.018