Difference between revisions of "Part:BBa K5036050"

 
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<partinfo>BBa_K5036050 short</partinfo>
 
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Our engineered switch contains NSP3 protein, which is linked to a nanobody that specifically recognizes MMP9. MMP9 is an enzyme that is elevated inside cells during injury. When injury occurs, the increased MMP9 binds to the nanobody, triggering a change in the switch's structure. This structural change causes a specific mRNA molecule to be transformed into a circular form, which promotes its translation into YAP.
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==Part Description==
  
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Our engineered switch contains a nanobody that recognizes MMP9, an enzyme elevated in injured cells. This nanobody is linked to NSP3 protein, which is attached to the cap from the other end.
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==Usage==
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Our TID device contains NSP3 protein linked to specific sensors (nanobodies) that detect MMP9, a protein elevated within cells after tissue injury. TID switches from off state to on state when MMP9 is present in the cell cytoplasm. This triggers the circularization of YAP mRNA, which is essential for protein production
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/5036/parts/nsp3-mmp9-nb.png">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of NSP3-MMP9 Nanobody which is attached to the cap of our switch
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.  </span></p></div></html>
  
  

Revision as of 16:26, 22 September 2024


MMP9 Nanobody3- NSP3

Part Description

Our engineered switch contains a nanobody that recognizes MMP9, an enzyme elevated in injured cells. This nanobody is linked to NSP3 protein, which is attached to the cap from the other end.

Usage

Our TID device contains NSP3 protein linked to specific sensors (nanobodies) that detect MMP9, a protein elevated within cells after tissue injury. TID switches from off state to on state when MMP9 is present in the cell cytoplasm. This triggers the circularization of YAP mRNA, which is essential for protein production

this figure illustrates the structure of NSP3-MMP9 Nanobody which is attached to the cap of our switch .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]