Difference between revisions of "Part:BBa K5036046"

 
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<partinfo>BBa_K5036046 short</partinfo>
 
<partinfo>BBa_K5036046 short</partinfo>
  
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In our engineered switch, we've incorporated MCP protein into a nanobody that specifically recognizes MMP9. MMP9 is an enzyme that is elevated inside cells during injury. When injury occurs, the increased MMP9 binds to the nanobody, triggering a change in the switch's structure. This structural change causes a specific mRNA molecule to be transformed into a circular form, which promotes its translation into a protein called YAP.
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==Part Description==
  
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Our engineered switch contains a nanobody that recognizes MMP9, an enzyme elevated in injured cells. This nanobody is linked to MCP protein, which is attached to MS2 from the other end because of its high affinity for MS2.
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==Usage==
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Our TID device contains MCP protein linked to specific sensors (nanobodies) that detect MMP9, a protein elevated within cells after tissue injury. TID switches from off state to on state when MMP9 is present in the cell cytoplasm. This triggers the circularization of YAP mRNA, which is essential for protein production
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/5036/parts/mcp-mmp9-nb.png">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of MCP-MMP9 Nanobody which is attached to our switch
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.  </span></p></div></html>
  
  

Revision as of 15:56, 22 September 2024


MCP-MMP9 Nanobody2

Part Description

Our engineered switch contains a nanobody that recognizes MMP9, an enzyme elevated in injured cells. This nanobody is linked to MCP protein, which is attached to MS2 from the other end because of its high affinity for MS2.

Usage

Our TID device contains MCP protein linked to specific sensors (nanobodies) that detect MMP9, a protein elevated within cells after tissue injury. TID switches from off state to on state when MMP9 is present in the cell cytoplasm. This triggers the circularization of YAP mRNA, which is essential for protein production

this figure illustrates the structure of MCP-MMP9 Nanobody which is attached to our switch .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]