Difference between revisions of "Part:BBa K5246011"

 
Line 3: Line 3:
 
<partinfo>BBa_K5246011 short</partinfo>
 
<partinfo>BBa_K5246011 short</partinfo>
  
−
HfsK gene from Caulobacter crescentus encodes an acetyltransferase protein of 359aa. HfsK is c-di-GMP effector involved in holdfast biogenesis. Cells lacking HfsK form highly malleable holdfast structures with reduced adhesive strength that cannot support surface colonization. HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle but is transfered to cytosol in the process of holdfast synthesis. HfsK deacetylates N-acetyl-glucosamine from the holfast which results in better adhesive properties.
+
===Introduction===
 +
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
HfsK gene from Caulobacter crescentus encodes an acetyltransferase protein of 359aa. HfsK is c-di-GMP effector involved in holdfast biogenesis. Cells lacking HfsK form highly malleable holdfast structures with reduced adhesive strength that cannot support surface colonization. HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle but is transfered to cytosol in the process of holdfast synthesis. HfsK deacetylates N-acetyl-glucosamine from the holfast which results in better adhesive properties.
  
−
<!-- -->
+
===Sequence and Features===
−
<span class='h3bb'>Sequence and Features</span>
+
 
<partinfo>BBa_K5246011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246011 SequenceAndFeatures</partinfo>
  
  
−
<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5246011 parameters</partinfo>
 
<partinfo>BBa_K5246011 parameters</partinfo>
−
<!-- -->
+
 
 +
===Experimental characterization===
 +
 
 +
 
 +
===References===

Revision as of 14:14, 22 September 2024


CB2/CB2A HfsK Acetyltransferase

Introduction

Usage and Biology

HfsK gene from Caulobacter crescentus encodes an acetyltransferase protein of 359aa. HfsK is c-di-GMP effector involved in holdfast biogenesis. Cells lacking HfsK form highly malleable holdfast structures with reduced adhesive strength that cannot support surface colonization. HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle but is transfered to cytosol in the process of holdfast synthesis. HfsK deacetylates N-acetyl-glucosamine from the holfast which results in better adhesive properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References