Difference between revisions of "Part:BBa K177024"

(→‎Usage and Biology)
(→‎Usage and Biology)
 
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When tested strains transformed only with this brick are incubated without IPTG and green fluorescence is observed then the bacteria are lacI<sup>-</sup>.
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When tested strains transformed only with this brick are incubated without IPTG or lactose and green fluorescence is observed then the bacteria are lacI<sup>-</sup>.
  
  

Latest revision as of 15:36, 21 October 2009

LacI detector device


This device was constructed as a test to determine which of the E. coli strains available were truly lacI-.


Usage and Biology

When tested strains transformed only with this brick are incubated without IPTG or lactose and green fluorescence is observed then the bacteria are lacI-.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 873