Difference between revisions of "Part:BBa K5226049"

 
 
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===Sequence and Features===
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<partinfo>BBa_K5226049 SequenceAndFeatures</partinfo>
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<br>
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===Introduction===
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One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to <b>build thermosensitive bio-switch</b> for <i>Halomonas</i> TD.We successfully built one <b>using ci857 regulatory protein</b>. And for better performance and broader range of usage, we <b>mutated CI857 and its mutant TCI-42 to see whether we could get one or several well-performed mutant with lower leakage and higher fold change</b>.
  
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===Usage and Biology===
 
===Usage and Biology===
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T64 is <b>a mutant of TCI-42</b>,a widely used mutant of cI from <i>bacteriophage</i> λ as a thermogenetic tool. <b>At 30℃ ,The cI857 dimer repressor binds in a cooperative form, by protein-protein interactions of their C-terminal domains that exhibits strong repression on R promoter. At 37℃, the dimer dissociates, So the R promoter works.After our verification by experiment, C27 has lower leakage and higher fold change than CI857.</b>
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<span class='h3bb'>Sequence and Features</span>
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Latest revision as of 12:26, 22 September 2024


TcI42-T64

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to build thermosensitive bio-switch for Halomonas TD.We successfully built one using ci857 regulatory protein. And for better performance and broader range of usage, we mutated CI857 and its mutant TCI-42 to see whether we could get one or several well-performed mutant with lower leakage and higher fold change.

Usage and Biology

T64 is a mutant of TCI-42,a widely used mutant of cI from bacteriophage λ as a thermogenetic tool. At 30℃ ,The cI857 dimer repressor binds in a cooperative form, by protein-protein interactions of their C-terminal domains that exhibits strong repression on R promoter. At 37℃, the dimer dissociates, So the R promoter works.After our verification by experiment, C27 has lower leakage and higher fold change than CI857.