Difference between revisions of "Part:BBa K177025"
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− | When tested strains transformed only with this brick are incubated | + | When tested strains transformed only with this brick are incubated without arabinose and green fluorescence is observed then the bacteria are araC<sup>-</sup>. |
Latest revision as of 15:34, 21 October 2009
GFP under araC promotor
This device was constructed as a test to determine which of the E. coli strains available were truly araC-.
Usage and Biology
When tested strains transformed only with this brick are incubated without arabinose and green fluorescence is observed then the bacteria are araC-.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 294
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 125
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1013
Illegal SapI site found at 107