Difference between revisions of "Part:BBa K5226045"

 
 
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<partinfo>BBa_K5226045 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K5226045 SequenceAndFeatures</partinfo>
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===Introduction===
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One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> strains that can <b>produce tyrian purple</b>. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.
  
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===Usage and Biology===
 
===Usage and Biology===
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MaFMO is a kind of <b>flavin-containing monooxygenase</b>. The protein sequence is from <i>Methylophaga aminisulfidivorans</i>. This protein <b>catalyzes the conversion 6-Br-indole into 6,6'-dibromoindigo (6BrIG, also known as tyrian purple)</b>.
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<span class='h3bb'>Sequence and Features</span>
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Latest revision as of 12:04, 22 September 2024


fmo

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 484
    Illegal AgeI site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.

Usage and Biology

MaFMO is a kind of flavin-containing monooxygenase. The protein sequence is from Methylophaga aminisulfidivorans. This protein catalyzes the conversion 6-Br-indole into 6,6'-dibromoindigo (6BrIG, also known as tyrian purple).