Difference between revisions of "Part:BBa K5226044"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5226044 short</partinfo> | <partinfo>BBa_K5226044 short</partinfo> | ||
+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K5226044 SequenceAndFeatures</partinfo> | ||
+ | <br> | ||
+ | ===Introduction=== | ||
+ | One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> strains that can <b>produce tyrian purple</b>. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | TnaA is a kind of <b>tryptophanase</b>. The protein sequence is from <i>Escherichia. coli</i> BL21(DE3). This protein <b>catalyzes the conversion of 6-Br-Trp into 6-bromoindole (6-Br-indole)</b>. | |
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Latest revision as of 12:01, 22 September 2024
tnaA
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 596
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 596
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 596
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 596
Illegal AgeI site found at 1219 - 1000COMPATIBLE WITH RFC[1000]
Introduction
One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.
Usage and Biology
TnaA is a kind of tryptophanase. The protein sequence is from Escherichia. coli BL21(DE3). This protein catalyzes the conversion of 6-Br-Trp into 6-bromoindole (6-Br-indole).