Difference between revisions of "Part:BBa K5321005:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | Gene synthesis should be performed to obtain this coding sequence. | ||
===Source=== | ===Source=== | ||
| − | + | Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli. | |
===References=== | ===References=== | ||
Latest revision as of 10:28, 22 September 2024
Tobacco etch virus protease (TEVp), codon optimized for E. coli, 6x His tagged
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 280
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 280
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 280
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 280
Illegal AgeI site found at 744 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gene synthesis should be performed to obtain this coding sequence.
Source
Nucleotide sequence reconstruction was performed using the amino acid sequences given in the Supplementary Material of Tina Fink et al., 2019, optimized for codon optimization in E. coli.