Difference between revisions of "Part:BBa K257002:Design"

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===Design Notes===
 
===Design Notes===
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For fusion of proteins to the C-terminal extremity of ClyA using the silver fusion standard RFC23. We also need a poly gly linker at the C-terminal extremity.
  
 
===Source===
 
===Source===

Revision as of 15:32, 21 October 2009

ClyA_Fusion_C-Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For fusion of proteins to the C-terminal extremity of ClyA using the silver fusion standard RFC23. We also need a poly gly linker at the C-terminal extremity.

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References