Difference between revisions of "Part:BBa K245022"

 
 
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<partinfo>BBa_K245022 short</partinfo>
 
<partinfo>BBa_K245022 short</partinfo>
  
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The sequence of this part codes N-terminal of yellow flourescent protein (YFP). Flourescence of the protein can only be detected when combined with another part of YFP protein that is not included in this part but can be found as seperate part in the Registry (cYFP: BBa_K245021). This feature proves useful when interactions between proteins are under inspection. Based on presence or absence of flourescence and position of nYFP and cYFP domains in studied polypeptides one can deduct how these polypeptides assemble in larger structures and/or when this proces takes place.
  
 
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Latest revision as of 15:31, 21 October 2009

nYFP

The sequence of this part codes N-terminal of yellow flourescent protein (YFP). Flourescence of the protein can only be detected when combined with another part of YFP protein that is not included in this part but can be found as seperate part in the Registry (cYFP: BBa_K245021). This feature proves useful when interactions between proteins are under inspection. Based on presence or absence of flourescence and position of nYFP and cYFP domains in studied polypeptides one can deduct how these polypeptides assemble in larger structures and/or when this proces takes place.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]