Difference between revisions of "Part:BBa K191004"

 
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<partinfo>BBa_K191004 short</partinfo>
 
<partinfo>BBa_K191004 short</partinfo>
  
 
Readout 1, RFP's transcription controlled by TRP operon
 
Readout 1, RFP's transcription controlled by TRP operon
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Our first read-out system consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophane biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophane.
  
 
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Revision as of 15:29, 21 October 2009

TRP promoter - RBS - RFP - Term

Readout 1, RFP's transcription controlled by TRP operon

Our first read-out system consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophane biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
    Illegal AgeI site found at 666
    Illegal AgeI site found at 778
  • 1000
    COMPATIBLE WITH RFC[1000]