Difference between revisions of "Part:BBa K5374008:Design"

 
 
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<partinfo>BBa_K5374008 short</partinfo>
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<partinfo>BBa_K5374008 SequenceAndFeatures</partinfo>
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===Design Notes===
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The recombinant BMP-4 sequence was codon-optimized for expression in E. coli. Additionally, its structure was carefully maintained to preserve the protein’s bioactivity in osteogenic pathways.
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===Source===
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BMP-4 is derived from human genes but can be produced recombinantly using E. coli or other systems.
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===References===

Latest revision as of 05:32, 22 September 2024


BMP-4 (Bone Morphogenetic Protein-4).BMP-4 is a signaling protein that plays a key role in bone form


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 61
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The recombinant BMP-4 sequence was codon-optimized for expression in E. coli. Additionally, its structure was carefully maintained to preserve the protein’s bioactivity in osteogenic pathways.


Source

BMP-4 is derived from human genes but can be produced recombinantly using E. coli or other systems.

References